Either solution is generally considered suitable as a simple stain to observe morphology.
R17. Disinfectants (for preparation of canned foods for microbiological analysis)
1. Alcoholic solution of iodine Potassium iodide 10 g Iodine 10 g Ethanol (70%) 500 ml
2. Sodium hypochlorite solution *Sodium hypochlorite 5.0-5.25 g Distilled water 100 ml
*Laundry bleach, which is 5.25% sodium hypochlorite (NaOCl), may be used.
R18. Dulbecco's Phosphate-Buffered Saline (DPBS) NaCl 8.0 g KCl 200 mg Na2HPO4 1.15 g KH2PO4 200 mg CaCl2 100 mg MgCl2·6H2O 100 mg Distilled water 1 liter
Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.2.