R30. Hippurate Solution, 1%
Dissolve 0.1 g sodium hippurate in 10 ml distilled water. Filter- sterilize and store refrigerated or in 0.4 ml aliquots at -20°C. Commercial preparations are also available.
R31. Horseradish Peroxidase (color development solution)
Solution A (horseradish) HRP color development reagent 60 mg Ice cold methanol 20 ml
Mix to dissolve. Protect from light and prepare fresh.
Solution B Ice cold hydrogen peroxide, 30% 60 µl Tris-buffered saline 100 ml
Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.
R32. 1 N Hydrochloric Acid HCl (concentrated) 89 ml Distilled water to make 1 liter
R33. Kovacs' Reagent p-Dimethylaminobenzaldehyde 5 g