reagent has been added within 3 min, since reagent is toxic to organisms.
R40. Peptone Diluent, 0.1% Peptone 1 g Distilled water 1 liter
Autoclave 15 min at 121°C. Final pH, 7.0 + 0.2.
R41. O.01 M Phosphate-Buffered Saline (pH 7.5)Stock solution (0.1 M) Na2HPO4 (anhydrous) 12.0 g NaH2PO4·H20 2.2 g NaCl 85.0 g Distilled water 1 liter
Dissolve ingredients in distilled water and bring volume to 1 liter. Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary.
R42. 0.02 M Phosphate Saline Buffer (PH 7.3-7.4) Prepare stock solutions of 0.2 M mono- and disodium phosphate in 8.5% salt solutions and dilute 1:10 for preparation of 0.02 M phosphate saline buffer.
Stock solution 1 Sodium phosphate dibasic anhydrous Na2HPO4 (anhydrous) (reagent grade) 28.4 g