Add powdered ingredients to distilled water and mix by rotating or stirring the flask.
Stir while heating if heating is required to dissolve the medium.
Autoclave the medium when the ingredients are dissolved.
Sterilization and sterility testing
Always sterilize a medium at the correct temperature and for the correct length of time as instructed in the method of preparation. Methods used to sterilize culture media
. Steaming to 100 OC
Autoclaving is used to sterilize most agar and fluid culture media.
B) Steaming at 100 OC It is used to sterilize media containing ingredients that would be inactivated at temperature over 100 OC and re-melt previously bottled sterile agar media.
It is used to sterilize additives that are heat-sensitive and can not be autoclaved.