Inoculation of culture media
When inoculating culture media, an aseptic technique must be used to prevent contamination of specimens and culture media, and laboratory worker and the environment.
Aseptic technique during inoculation of culture media
Decontaminate the workbench before and after the work of the day. Use facemask and gloves during handling highly infectious specimens. Flame sterilize wire loops, straight wires, and metal forceps before and after use. Flame the neck of specimen and culture bottles, and tubes after removing and before replacing caps and plugs.
Sterlizing the inoculating loop with flame
Inoculating the fluid media with sterilized loop
Aseptic inoculation technique