Inoculation of media in petridishes The inoculation of media in petridishes is named as ‘plating out’ or ‘looping out’. Before inoculating a plate of culture media, dry the surface of the media by incubating at 37 OC for 30 minutes. To inoculate a plate, apply the inoculum to a small area of the plate (‘the well’) using sterile wire loop and then spread and thin out the inoculum to ensure single colony growth.
Fig. 1.7 Methods of ioculating solid culture media in petridishes