Inoculation of butt and slant media To inoculate butt and slant media, use a sterile straight wire to stab into the butt and then streak the slant in a zigzag pattern.
Inoculation of slant media To inoculate slant media, use a straight wire to streak the inoculum down the center of the slant and then spread the inoculum in a zigzag pattern.
Fig. 1.8 Inoculation of slant and bath media
Inoculation of stab media To inoculate stab media, use a straight wire to stab through the center of the medium and withdraw the wire along the line of inoculum.