Fig. 1.9 Inoculation of slant media
Inoculation of fluid media To inoculate fluid media, use straight wire or wire loops.
Incubation of cultures Inoculated media should be incubated as soon as possible. Optimal temperature, humidity and gaseous atmosphere should be provided for microorganisms to grow best. The temperature selected for routine culturing is 35-37 OC. Some pathogens require CO2-enriched atmosphere to grow in culture media, and the simplest way to provide CO2-enriched atmosphere is to enclose a lighted candle in an airtight jar which provides 3-5% CO2 by the time the candle is extinguished.
Anaerobic atmosphere is essential for the growth of strict anaerobes, and the techniques for obtaining anaerobic conditions are the following:
. Anaerobic jar with a gas generating kit. . Reducing agents in culture media.