. Observe for changes in color every 30 min.
Results: . Change in color of medium from pink to white or pale is suggestive of enterococci CAMP test (Christie, Atkins , Munich Paterson ) Principle: S.agalaciae produce protein named as camp factor, which interacts with staphylococci β- hemolysin on sheep red blood cell. Method: . Streak S.aureus isolate across sheep blood agar plate. . Inoculate the test bacteria at right angle to staphylococci with out touching it. . Incubate over night at 35-37 Oc. . Formation of an arrow-head shaped area of hemolysis indicates interaction of camp factor with staphylococci hemolysin.
Bacitracin test Principle: Streptococcus pyogenes is sensitive to bacitracin
but other kinds of streptocci are resistant to bacitracin.
. Streak a blood agar plate with the isolated organism. . Place bacitracin disc in the streaked area. . Incubate the plate for 24 hours at 37 0c.