Catalase acts as a catalyst in the breakdown of hydrogen peroxide to oxygen and water.
An organism is tested for catalase production by bringing it into contact with hydrogen peroxide. Bubbles of oxygen are released if the organism is a catalase producer. The culture should not be more than 24 hours old.
Care must be taken if testing an organism cultured on a medium containing blood because catalase is present in red cells. If any of the blood agar is removed with the colony, a false positive reaction will occur. It is usually recommended, therefore, that catalase testing be performed from a blood free culture medium such as nutrient agar.
Required a. Hydrogen peroxide, 3% H2O2 Note: Shaking the reagent before use will help to expel any dissolved oxygen. False positive reactions may occur if the hydrogen peroxide contains dissolved oxygen.
Pour 2-3ml of the hydrogen peroxide solution into a test