Using a sterile wooden stick or a glass rod, remove a good growth of the test organism and immerse it in the hydrogen peroxide solution.
Note: A nichrome wire loop must not be used because this may give a false positive reaction.
Look for immediate bubbling.
Results Active bubbling ----------------- Positive test Catalase produced Negative test No catalase produced Note: if the organism has been cultured on an agar slope, pour about 1ml of the hydrogen peroxide solution over a good growth of the organism, and look for the release of bubbles. No release of bubbles ----------
Caution: performing the test on a slide is not recommended because of the risk of contamination from active bubbling. If the rapid slide technique is used, the hydrogen peroxide solution should be added to the organism suspension after placing the slide in a petridish. The dish should then be covered immediately, and the preparation observed for bubbling through the lid.
Positive catalase control: Staphylococcus species. Negative catalase control: Streptococcus species.