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Results Blackening ----------------------------- No blackening ------------------------- produced.

Positive test H2S produced

Negative

test

No

H2S

Controls Positive hydrogen sulphide control: proteus vulgaris Negative hydrogen sulphide control: shigella species

INDOLE TEST Testing for indole production is important in the identification of enterobacteria. Most sftrains of E.coli, P.vulgaris, P.rettgeri, M.morganll, and providencia species break down the amino acid tryptophan with the release of indole.

Principle The test organism is cultured in a medium which contains tryptophan. Indole production is detected by Kovac’s or Ehrlich’s reagent which contains 4(P)-dimethylaminobenzaldehyde. This reacts with the indole to produce a red coloured compound. In the following method the use of the combined motility indole urea (MIU) medium is described. A Kovac’s ragent paper strip is inserted in the neck of the tube, and indole production is indicated by a reddening of the strip. Indole is a volatile substance (easily vaporized). The tube must be well stoppered during incubation. The indole test can also be performed by culturing the organism in tryptone water or peptone water containing tryptophan, and

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