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Note:  One survival surgical procedure followed by a non-recovery procedure is not included in this category.

1)Describe the institutional policy(ies) regarding multiple survival surgery (major or minor) on a single animal.

2)Describe the procedure for approving multiple survival surgery (major or minor) and the criteria used to determine the potential impact on the animals’ well-being.

3)Summarize the protocols currently approved that involve multiple major survival surgical procedures and the time allowed between procedures on the same animal. Describe the method of institutional monitoring.

v.Food and Fluid Regulation [Guide, pp. 30-31]

1)Describe experimental situations that require food and/or fluid regulation. Note: This does not include pre-surgical fast.  List title of the experiment(s), justification, species involved, and length and type of food/fluid regulation.

2)Describe animal health monitoring procedures and frequency (e.g., body weight, blood urea nitrogen, urine/fecal output, food/fluid consumed).

3)Describe methods of ensuring adequate nutrition and hydration during the regulated period.

vi.Use of Non-Pharmaceutical-Grade Drugs and Other Substances [Guide, p. 31]

Describe the rationale and consideration given by the IACUC/OB for use of non-pharmaceutical grade drugs or other substances, if applicable.

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