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DL-LACTONE CAS N°:79-50-5 - page 50 / 113

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OECD SIDS 3. ENVIRONMENTAL FATE AND PATHWAYS

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

degradation percentages achieved. Therefor qualitative and semi-quantitative descript The BOD in both 100 and 1000 mg/l test ves the blank sludge BOD for 6 respectively 8 biodegradation (test substance BOD minus b in both concentrations. In the 100-mg/l vessel, the net BOD attain as measured on the printout of three-quart BOD within 6 days from the start of net bi total day 12. At the end of the test at 21

e, only a ion can be given. sels remained below days. Then net lank BOD) took off

ed an estimated rate ers of the final net odegradation, ie, on

days, the

biodegradability of the 100-mg/l handwriting as "91%". In the 1000-mg/l vessel, the net rate as measured on the printout final net BOD within 9 days from biodegradation, ie, on total day 21 days, the biodegradability of

vessel is marked in

BOD attained an estimated of three-quarters of the the start of net 17. At the end of the test at the 1000-mg/l vessel is

Test substance: Conclusion:

Reliability:

marked in handwriting as "80% was still going upwards steep dl-Lactone from Roche, "pure" Pure dl-lactone was well inhe respirometric test at both 10 concentration. In contrast to initial lag phase was seen wi activated sludge blank that l

days (2)

for the higher test conc valid with restrictions

". The BOD curve for this vessel ly. , not otherwise characterised. rently biodegradable in a

  • 0

    and 1000 mg/l starting

the DOC Zahn-Wellens test, an th a reduced BOD compared to the asted 6 days for the lower and 8 entration.

Biodegradability

assessment

f

not

GLP

but

highly

standardis

rom the in-house wastewater lab, ed test in a professional

Flag: 10-MAR-2005

laboratory

with

Critical

study

many

years

of

for

SIDS

endpo

experience. int

(24)

(25)

Type: Inoculum:

Concentration: Contact time: Degradation: Result: Deg. product:

aerobic other: activated sludge, mixed domestic and industrial, non-adapted 100 mg/l related to Test substance 15 day(s) ca. 82 % after 13 day(s) inherently biodegradable not measured

Method:

Year: GLP: Test substance:

other: comparable to OECD Guide-line 302 C 1983 no as prescribed by 1.1 - 1.4

Method:

A standard inherent degradation test was performed with pure dl-lactone from lab production in a Sapromat respirometer in a test setup corresponding to OECD 302C, except that the sludge used as an inoculum was a 1:1 (dry weight) mixture of non-adapted activated sludge from one single small municipal sewage works and of activated sludge from the Roche in-house pilot sewage plant, which receives a flow-proprtional sample of the industrial wastewater. The only test concentration was 100 mg dl-lactone/l. Activated sludge concentration is explicitly stated on the net degradation printout as 200 mg (dry weight)/l. Degradation was determined by oxygen consumption in test substance vessels minus the oxygen consumption of a blank sludge vessel divided by the COD for

50

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