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DL-LACTONE CAS N°:79-50-5 - page 58 / 113

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OECD SIDS 4. ECOTOXICITY

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

AQUATIC ORGANISMS

4.1 Acute/Prolonged Toxicity to Fish

Type: Species:

semistatic Cyprinus carpio

(Fish, fresh water)

Exposure period:

96 hour(s)

Unit: NOEC: LC0: LC50: Limit Test:

mg/l

Analytical monitoring: yes

ca. 140 - measured/nominal ca. 140 - measured/nominal

  • >

    140 - calculated

yes

Method:

Year: GLP: Test substa

nce:

OECD Guide-line 203 1999 yes as prescribed by 1.1 - 1.4 "Fish, Acute Toxicity Test"

Method:

The study was performed according to OECD Guideline 203 under GLP. Fish: Juvenile carp from Zodiac, "De Haar Vissen" company, Wageningen, the Netherlands; acclimation for at least 12 days. Fish were healthy with less than 5% mortality in the 7 days preceding the test. Fish used in the range-finding test were 3.3±0.19 cm long and weighed 1.25±0.17 g, fish in the main test were 2.88±0.21 cm and weighed 0.68±0.14 g. Fish were fed daily with Trouvit until two days before testing. Medium: ISO medium, full composition in report, formulated with Milli-Ro water. Hardness and pH were measured before use in the test; temperature was measured daily; pH, nitrate, nitrite and ammonia were measured once a week. Test solutions: As dl-lactone was shown to be hydrolytically unstable, a semi-static test scheme was followed. Tests solutions were made daily by dissolving the exact amount of test substance by careful mixing. In the range-finder the lower concentrations were made up by dilution with test medium. Test media were not aerated during the test. Procedure: 10-litre all-glass tanks filled to 6 l. 7 fish per concentration and control, introduced into the respective tank directly after preparation of the medium. Daily medium exchange for fresh test solutions. Photoperiod 16 h light, 8 h dark. Range-finding test: A range finder was performed with 4 concentrations of 100, 10, 1 and 0.1 mg/l and three fish per concentration. Main test: Based on no observed effects in all concentrations in the pretest, a limit test with 7 fish at 100 mg dl-lactone/l and 7 fish in the medium control was performed. Measurement and recordings: Fish were observed at approximately 2, 24, 48, 72 and 96 hours after first introduction into test media. Fish length and weight were measured in 10 specimens prior to the start of the test. Dissolved oxygen and pH were measured daily in all vessels, beginning at time = 0. Temperature was measured at

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