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DL-LACTONE CAS N°:79-50-5 - page 59 / 113

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OECD SIDS 4. ECOTOXICITY

Result:

Test substance:

Conclusion:

Reliability:

Flag: 24-AUG-2004

Type: Species: Exposure period: Unit: LC50:

Method: GLP: Test substance:

Remark:

Result:

Test substance:

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

the beginning and at the end of the test in one control vessel. Sampling and analytics: Samples from the 100-mg/l and bank tanks were taken at t = 0 and t = 24 h from the approximate centre of the tank and analysed by HPLC wihtout storage. HPLC conditions

Column

Mobile ph

ase

LiChrospher 100RP-18, 250*4 (i.d.) mm, d(rho)= 5 µm (Merck, Germany) 20/80/0.1 (v/v/v) acetonitrile/Milli-Q

Flow Detection

Interface Monit mas

ses

water/formic acid 1 ml/min SCIEX MSMS system API-300 mass spectrometer (Perkin Elmer, USA) ion-spray, positive mode MRM m/z 131.3 --> 113.0 (test substance)

Inject volume Int standard

MRM m/z 127.2 --> 98.9 (in 100 µl 4-hydroxy-6-methyl-2-pyron Aldrich, USA)

ternal standard)

e (98%, Sigma-

No visible effects of dl-lactone exposure the range finder at all concentrations an test. dl-Lactone was completely soluble at all tested. The highest concentration in the mg/l nominal concentration and analysed a hours. The concentration in the main test mg/l at t = 0, 145 mg/l at t = 24 h in th mg/l at t = 24 hours in the fresh medium. dl-Lactone from Roche Dalry, batch 805046 according to certificate of analysis. dl-Lactone was not acutely toxic to carp OECD test at 140 mg/l average concentrati and LC0 was 140 mg/l and the LC50 could n no single fish died. Moreover, dl-lactone (>80%) stable over the medium exchange pe

were noted, both in d in the main limit

concentrations range-finder was 100 t 93.2 mg/l after 24

was analysed at 142 e old water and 138

, purity 100.0%

in a semi-static on. The 96-hour NOEC ot be determined as

was sufficiently riod of 24 hours.

(1) OECD

valid without restriction test under GLP with analytical

confi

rmation.

Critical

study

for

SIDS

endpoint

(8)

static Leuciscus idus 96 hour(s)

(Fish, fresh water)

mg/l

Analytical monitoring: no data

  • >

    100 - measured/nominal

other: DIN 38412 part 15, static no data other TS

No further data on method, year or GLP due to the characteristics of the source, a safety data sheet. The result is being cited as supportive evidence. In a safety data sheet for D-pantolactone (one of the two isomers in dl-lactone) from BASF Company in Germany, the acute toxicity of D-pantolactone to fish is stated as >100 mg/l (nominal concentration). Crystalline D-pantolactone, CAS 599-04-2, purity 99% (communication from BASF, 26-Aug-2004).

UNEP PUBLICATIONS

59

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