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DL-LACTONE CAS N°:79-50-5 - page 60 / 113

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OECD SIDS 4. ECOTOXICITY

Reliability:

30-JUN-2005

4.2 Acute Toxicity

Type: Species: Exposure period: Unit: NOEC: EC50: Limit Test:

Method:

Year: GLP: Test substance:

Method:

60

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

(4) The

not assignable safety data sheet

received

source safety

without any experimental data sheets.

from

BASF

details,

is as

only a condensed is typical for

(3)

to Aquatic Invertebrates

semistatic Daphnia magna

(Crustacea)

48 hour(s) mg/l

Analytical monitoring: yes

ca. 130 - measured/nominal

  • >

    130 - calculated

yes

OECD Guide-line 202 1999 yes as prescribed by 1.1 - 1.4

The study was performed according to OECD Guideline 202 under GLP. Daphnia: Young daphnia with an age of <24 hours from the NOTOX breeding stock were used for the test. Each breeding batch was started with young animals of <3 d by placing approximately 250 of them into 10 l of medium in an all-glass culture vessel. After 7 days cultivation, hylf the medium was echanged twic weekly. Daphnids were fed daily with a suspension of fresh-water algae. Temeprature was kept in the range of 18-22 °C, constant within 1 °C. Cultures were used up to a maximum age of 4 weeks, then new batches were started. Medium: M7 medium as prescribed by Elendt-Schneider, full composition in report, formulated with Milli-Ro water. Test solutions: As dl-lactone was shown to be hydrolytically unstable, a semi-static test scheme was followed. Tests solutions were made daily by dissolving the exact amount of test substance by careful mixing. In the range-finder the lower concentrations were made up by dilution with test medium. Test media were not aerated during the test. Procedure: 100-millilitre all-glass vessels filled up to the 80-ml mark

were used. and blanks

10 daphnids were tested

were used per in duplicate.

vessel, concentrations

Daphnids

were

introduced

into

the

respective

vessel

directly

after

preparation

of

the

medium. Daily medium exchange for fresh test solutions. Photoperiod 16 h light, 8 h dark. Range-finding test: A range finder was performed with 4 concentrations of 100, 1 and 0.1 mg/l with 10 daphnia per concentration. Main test: Based on no observed effects in all concentrations in the pretest, a limit test with 2 x 10 daphnids at 100 mg dl-lactone/l and 2 X 10 daphnids in the medium control was performed. Measurement and recordings:

10,

UNEP PUBLICATIONS

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