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DL-LACTONE CAS N°:79-50-5 - page 61 / 113

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OECD SIDS 4. ECOTOXICITY

Result:

Test substance:

Conclusion:

Reliability:

Flag: 24-AUG-2004

Type: Species: Exposure period: Unit: EC50:

Method: GLP: Test substance:

Remark:

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

Daphnids were observed at at the b after first introduction into test pH were measured daily in all vess Temperature was measured at the be the test in one control vessel. Sampling and analytics: Samples from the 100-mg/l and blan and t = 24 h from the freshly prep old solutions from the approximate analysed by HPLC without storage. HPLC conditions

eginning, at 24 and 48hours

media. Dissolved oxygen and els, beginning at time = 0. ginning and at the end of

k vesselwere taken at t = 0 ared solutions and from the centre of the vessel and

Column

Mobile ph

ase

LiChrospher 100RP-18, 250*4 (i.d.) mm, d(rho)= 5 µm (Merck, Germany) 20/80/0.1 (v/v/v) acetonitrile/Milli-Q

Flow Detection

Interface

water/formic acid 1 ml/min SCIEX MSMS system API-300 mass spectrometer (Perkin Elmer, USA) ion-spray, positive mode

Monit masses

Inject volume Int standard

MRM m/z 131.3 --> 113.0 (test substance) MRM m/z 127.2 --> 98.9 (internal standard) 100 µl 4-hydroxy-6-methyl-2-pyrone (98%, Sigma- Aldrich, USA)

No visible effects in daphnids of dl-lactone exposure were noted, both in the range finder at all concentrations and in the main limit test. dl-Lactone was completely soluble at all concentrations tested. The highest concentration in the range-finder was 100 mg/l nominal concentration and analysed at 93.2 mg/l after 24 hours (same medium as used for fish test). The concentration in the main test was analysed at 144 mg/l at t = 0, 123 mg/l at t = 24 h in the old water and 138 mg/l at t = 24 hours in the fresh medium. dl-Lactone from Roche Dalry, batch 805046, purity 100.0% according to certificate of analysis. dl-Lactone was not acutely toxic to daphnids in a semi-static OECD test at 130 mg/l average concentration. The 48-hour NOEC was 130 mg/l and the EC50 could not be determined as no single

daphnids became immobilised. Moreover, dl-lactone was

sufficiently (>80%) stable over the 24 hours.

medium exchange

period

of

(1) OECD

valid without restriction test under GLP with analytical

confirmation.

Critical

study

for

SIDS

endpoint

(35)

static Daphnia magna 48 hour(s)

(Crustacea)

mg/l

Analytical monitoring: no data

  • >

    100 - measured/nominal

other: DIN 38412 part 11, static no data other TS

No further data on method, year or GLP due to the characteristics of the source, a safety data sheet. The result is being cited as supportive evidence.

UNEP PUBLICATIONS

61

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