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DL-LACTONE CAS N°:79-50-5 - page 62 / 113

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OECD SIDS 4. ECOTOXICITY

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

Result:

Test substance:

Reliability:

In a safety dat isomers in dl-l toxicity of D-p (nominal concen Crystalline D-p (communication

(4)

not assign

The

safety data

source

without

safety

data she

a sheet for D-pantolactone (one of the two actone) from BASF Company in Germany, the acute antolactone to daphnia is stated as >100 mg/l tration). antolactone, CAS 599-04-2, purity 99% from BASF, 26-Aug-2004). able

sheet received from BASF is only a condensed any experimental details, as is typical for ets.

30-JUN-2005

(3)

4.3 Toxicity to Aquatic Plants e.g. Algae

Species: Endpoint:

Selenastrum capricornutum (Algae) other: biomass and growth rate

Exposure period:

72 hour(s)

Unit: NOEC: EbC50 : ErC50 : Limit Test:

mg/l

Analytical monitoring: yes

= 100 - measured/nominal

  • >

    100 - calculated

    • >

      100 - calculated

yes

Method:

Year: GLP: Test substa

nce:

OECD Guide-line 201 1999 yes as prescribed by 1.1 - 1.4 "Algae, Growth Inhibition Test"

Method:

The study was performed according to OECD Guideline 201 under GLP. Algae: Selenastrum capricornutum, strain CCAP 278/4, from NOTOX's own stock culture. This was originally started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light (4000-9000 lux) in a climate room at a temperature of 23±2 °C. Pre-culture: 4 days before the start of the test, cells from the algal stock culture were inoculated in culture medium at a cell density of 2*E+4 cells/ml. The pre-culture was maintained under the same conditions as used in the test. The cell density was meaured immediately before use. Medium: M-2 medium according to ISO standard "Algal growth inhibition test of November 1989, full composition in report, formulated with Milli-Ro water. Test solutions: Tests solutions were made up by dissolving the exact amount of test substance by careful mixing. In the range-finder the lower concentrations were made up by dilution with ISO M-2 medium. After preparation, volumes of 50 ml were added to each replicate vessel of the respective test concentrations. Subsequently, a volume of 0.118 ml of algal suspension was added, for which the cell density had previously been determined, so as to provide a densitiy of 10E+4 cells/ml at the start of the test. Test procedure: Test duration was 72 h, under continuous illumination using 30-W TLD "Gro-Lux" lamps (Sylvania) with a light intensity within the range of 3500-4000 lux. Test vessels were kept on a

62

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