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DL-LACTONE CAS N°:79-50-5 - page 80 / 113

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OECD SIDS 5. TOXICITY

80

DL-LACTONE

ID: 79-50-5 DATE: 18.01.2006

Homburg, Germany).

  • B)

    The test substance at a 5% concentration.

  • C)

    A 1:1 w/w mixture of the test substance, at twice the

concentration used in (B) and Freunds' Complete Adjuvant. Note: One of each pair was on each side of the midline and from cranial A) to caudal C). On day 3 the dermal reactions caused by the intradermal injections were assessed for irritation. On day 7 the scapular area between the injection sites was clipped and subsequently rubbed with 10% sodium-dodecyl-sulfate (SDS; Boom, Meppel, The Netherlands) in vaseline using a spatula. This concentration of SDS provokes a mild inflammatory reaction. On day 8 the 10% SDS treated area between the injection sites was treated with 0.5 ml of a 50% test substance concentration using a Metalline patch (2x3 cm) mounted on Medical tape, which was held in place with Micropore tape and subsequently

Coban elastic exposure, the water and the exposure were

bandage. The dressing was removed after 48 hours

skin cleaned of residual

dermal

reactions

caused

test substance using by the epidermal

assessed

for

irritation.

Induction - Control animals The control animals were treated as described for the experimental animals except that, instead of the test substance, vehicle alone was administered.

Challenge - All animals On day 22 one flank of all animals was clipped and treated by epidermal application of a 50% test substance concentration and the vehicle (0.1 ml each), using Patch Test Plasters (Curatest, Lohmann, Almere, The Netherlands). The patches were held in place with Micropore tape and subsequently Coban elastic bandage. The dressing was removed after 24 hours exposure and the skin cleaned of residual test substance and vehicle using water. The treated sites were assessed for challenge reactions 24 and 48 hours after removal of the dressing.

Observations Mortality/Viability: Twice daily Overt toxicity: At least once daily. Body weights: Prior to start and at termination of the study. Skin reactions: Skin reactions were graded according to the following numerical scoring systems. Furthermore, a description of all other (local) effects was recorded. Whenever necessary, the treated skin-areas were clipped at least 3 hours before the next skin reading to facilitate scoring. Grading Irritation Reactions*: Erythema and eschar formation: No erythema.....................................0 Slight erythema (barely perceptible)............1 Well-defined erythema...........................2 Moderate erythema...............................3 Severe erythema (beet redness) to slight eschar formation (injuries in depth)...................4

Oedema formation: No oedema..........

.............................0

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