X hits on this document

PDF document

Special ReviewS in ORnithOlOgy - page 3 / 12

43 views

0 shares

0 downloads

0 comments

3 / 12

6

a

tabLe 1. Next-generation sequencing technologies .

Pacific Biosciences

Helicos

Dover Systems

Illumina

Applied Biosystems

Roche

Company

www.pacificbiosciences. com/

www.helicosbio.com

www.polonator.org

www.illumina.com

www3.appliedbiosystems. com/AB_Home/index.htm

www.roche-applied- science.com

Website

Not yet announced

Helicos Genetic Analysis System

Polonator G.007

Genome Analyzer IIx

SOLiD 3 Plus System (Supported Oligonucle- otide Detection)

Genome Sequencer

FLX

Platform

  • speCiaL reviews in ornitHoLogy

NA (single-molecule sequencing)

SMRT technology (single- molecule synthesis)

10 test versions anticipated in early 2010, commercial version dependent on test results (anticipated in second half of 2010)

Anticipated: $600,000 $99

Projected: 72 Mbp, 80,000 reads

586 bp average; 2,805 bp longest read

NA (single-molecule sequencing)

Asynchronous extension using polymerase (single- molecule synthesis)

2008

$999,000 $18,000

28 Gbp; 400 million reads

25–50 bp, average 35 bp

Emulsion PCR of bead-bound oligos

Ligation (nonamers, single-base encoding)

2009

$170,000 $1,000

4–5 Gbp, 8–10 Gbp with enrichment; 150 million mapable reads

13 bp (26-bp paired- end reads)

Isothermal bridge amplification on flowcell

Reversible terminator using polymerase

2006

$600,000 $4,000–6,000 27.5–33 Gbp

100 bp

Emulsion PCR of bead- bound oligos

Ligation (octamers, dual-base encoding)

2007

$600,000 $3,000

c

60+ Gbp, >1 billion tags per run

50 bp

Emulsion PCR of bead-bound oligos

Pyrosequencing using polymerase

2005

List price $500,000 $5,000 0.5 Gbp

400–500 bp (titanium chemistry) or 250 bp (standard chemistry)

Method of sequencing feature generation

b

Sequencing chemistry

Availability

Machine cost Reagents cost per run

Capacity (base pairs) per run

Read length

10–15 min minimum

8 days

4 days

2.5–9.5 days

3.5 days

10 h; 2 h data processing

Run length

NA

NA 50

8 lanes per flow cell, 2 flow cells

90 min 8 flow cells

8

35 s 16

Cycle length Run subdivision

auk, voL. 127

(continued)

Application specific, as low as 50 pg

10 ng (fragment library); 5 μg for mate-paired reads; input DNA of 200–10,000 bp in length

500 ng (higher for titanium chemistry)

Input DNA requirements

Document info
Document views43
Page views43
Page last viewedFri Dec 09 06:12:14 UTC 2016
Pages12
Paragraphs493
Words9460

Comments