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January 2010

tabLe 1. Continued.

Pacific Biosciences

Helicos

Dover Systems

Illumina

Applied Biosystems

Roche

Company

  • speCiaL reviews in ornitHoLogy

<12 h No clonal amplification, low cost, speed of run, direct RNA sequencing

1 day

No clonal amplification, low error rate with dual pass

Cost of machine

Up to 10× improvement in throughput (no hardware change necessary) in 12–18 months; direct RNA sequencing (no cDNA intermediate) in the first half of 2010

Open-source model reduces cost and promotes development

Short read length

Rolling circle colonies (“rolonies”) to replace emulsion PCR; 48-bp reads (24 + 24); >100 Gbp in 2010

2 days No emulsion PCR

Medium read length, long run time

120-bp reads in early 2010, 250- bp paired-end reads in 2010

12 per flow cell

2–4.5 days

Increased accuracy due to dual base calls, homopolymer reads sequence-able, very high output (Gbp)

Short read length

100 GB and 1,200 B reads per run anticipated in February 2010

16 barcodes (with 8 plate divisions × 2 plates = 256 tags per run)

3–4 days Long read length

Unreliable determination of homopolymer regions and large repeats

2010: 1 kbp read length, GSjunior bench-top platform, simplified workflow including automation of emulsion PCR and reduction in sample preparation time

12 barcodes

Length of prep time Advantages

Disadvantages

Future developments

Platform-specific barcoding

Information in this table was personally communicated by agents of the companies or is from other publications where noted. See section of text on amplification-based sequencing for explanation. Millar et al. 2008.

a b c

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